|
KMID : 0604520150410030243
|
|
Journal of the Society of Cosmetic Scientists of Korea
2015 Volume.41 No. 3 p.243 ~ p.252
|
|
|
A Study on the Inhibition of Skin Pigmentation by Lobaric Acid as Protease Activated Receptor-2 Antagonist
|
|
Goo Jung-Hyun
Lee Ji-Eun
Myung Cheol-Hwan
Park Jong-Il
Hwang Jae-Sung
|
|
|
Abstract
|
|
|
|
Melanosome, the pigment granule in melanocyte, determines the color of skin when it moves into the keratinocyte. Inhibition of melanosome transfer from melanocyte to keratinocyte results in skin depigmentation. Protease activated receptor-2 (PAR-2) is involved in signal transduction systems via cell membrane and increases the melasome transfer when it is activated by cleavage of their extracellular amino acid sequence by trypsin or by a peptide such as SLIGKV. Here, we showed that lobaric acid inhibited PAR-2 activation and affected the mobilization of Ca2+. The uptake of fluorescent microspheres and isolated melanosomes from melan-a melanocytes to keratinocytes induced by SLIGKV were inhibited by lobaric acid. Also, confocal microscopy studies illustrated a decreased melanosome transfer to keratinocytes in melanocyte-keratinocyte co-culture system by lobaric acid. In addition, lobaric acid induced visible skin lightening effect in human skin tissue culture model, melanodermⓡ. Our data suggest that lobaric acid could be an effective skin lightening agent that works via regulation of phagocytic activity of keratinocytes.
|
|
|
KEYWORD
|
|
|
PAR-2, melanosome transfer, keratinocyte phagocytosis, skin lightening, lobaric acid
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|